Cryodissection of vessels of the human heart.
DOI:
https://doi.org/10.26641/1997-9665.2015.1.82-85Keywords:
cryodissection, coronary artery, the solution, freezingAbstract
Background. One of the most actual problems of applied morphology is the problem of demonstrativeness. In the educational process it is important to demonstrate the organs taken from the human body with all features of their structure preserved. The basic method of normal anatomy is a dissection of cadaveric material. It gives anatomical preparations demonstrating the structure of the human body. But classical dissection has certain difficulties: the complexity of layer-by-layer tissue separation and extraction of important anatomical structures. Currently for the manufacture of anatomical preparations a number of other methods are used: method of corrosion and polymeric embalming. However these techniques are time consuming, expensive, and also can cause damage to the structures of the heart during their extraction out of adipose tissue. Objective. To create a new method for the dissection of the human heart, allowing to reduce the time and to improve the quality of the preparations. Methods. We have prepared two solutions with different freezing temperature. Tissue which needed to be preserved (myocardium) was impregnated with solution №1. Tissue that need to be deleted (adipose tissue), impregnated with solution №2. After freezing the heart myocardium frizzes, but unfrozen adipose tissue could be easily separated. We examined 30 human hearts: 15 preparations by the classical dissection, 15 preparations with the help of cryodissection. Results. Preparation of hearts by the classical method took about 180 minutes, with the help of cryodissection – 30 minutes. Visualization of the coronary arteries and their branches after our method is better, myocardium is smooth, also preserve the natural color of the drug. Additionally, there is no contact of the researcher with harmful conservatives (for example formaldehyde). Conclusion. We have developed a method for dissection of cadaveric material, which improves the quality of anatomical preparations and reduces the time of their creation.
References
- Hilkevich SV, Volchkevich DA, authors; Vorobyev VV, editor. [Training anatomical preparations of heart, brain, eye: method of making, description]. Grodno: GrGMU; 2010. 35 p. Russian.
- Prives MG, author; Ginzburg VV, editor. [Methods for conservation of anatomical preparations]. MedGiz; 1956. 127 p. Russian.
- Yaroslavtsev BM, author; Znamenskiy MS, editor. [The anatomical technique]. Frunze; 1961. 436 p. Russian.
- Goncharov NI, Speranskiy LS, Krayushkin AI, DmitrienkoSV, authors; Valishin ES, editor. [Manual on preparation and production of anatomical preparations]. Moscow: Meditsinskaia kniga; 2002. 192 p. Russian.
- GayvoronskiyIV, Gayvoronskiy AI, Nichiporuk GI.[The polymeric embalming - innovative technology in the morphology]. In: [Proceedings of the Russian Forum ‘Pirogov’s surgical week’; 2010 Nov 24-28; St Petersburg]. Vestnik of St Petersburg State University: Series 11, Medicine. 2010;(Suppl):22-3. Russian.
Downloads
How to Cite
Issue
Section
License
Copyright (c) 2018 Morphologia
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world.
The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.
