The in vitro investigation of morphological changes of permanent teeth enamel surface under the influence of exogenous means for caries prevention containing different calcium compounds.
DOI:
https://doi.org/10.26641/1997-9665.2015.1.65-70Keywords:
enamel, permanent teeth, mineralization, casein phosphopeptide - amorphous calcium phosphate, calcium glycerophosphate, magnesium chloride, caries preventionAbstract
Background. Rationalizing of the choice of the most effective means for exogenous prevention, especially during secondary mineralization, will increase cariesresistance of tooth enamel. Objective - in vitro study of changes of just erupted permanent teeth enamel surface under the influence of exogenous means for caries prevention containing different calcium compounds. Methods. For the study we used 35 enamel samples of permanent teeth that had erupted within the same term (premolars of 11-13 years old children, less than 6 months after eruption). 5 samples were the control group before the experiment. 30 samples were randomly divided equally into three groups (10 samples in each group) and stored in boxes with artificial saliva. Enamel samples of the first group were treated with cream “TOOTH MOUSSE” (10% casein phosphopeptide - amorphous calcium phosphate), samples of the second group were treated with gel “R.O.C.S.® Medical minerals” (calcium glycerophosphate, magnesium chloride, xylitol). Samples of the first and second groups were treated at the beginning of the research and 3, 6 and 9 months later during 10 days, twice a day for 30 minutes. Samples of the third group were the control and were not treated. Investigation of the enamel surface was performed at the beginning of the experiment, after 6 and 12 months. The structure of the samples surface was studied at the equator zone with the scanning electron microscope. Results. At the beginning of the experiment signs of insufficient mineralization were observed on the surface of the enamel samples of the control group. After 12 months of treatment with cream with casein phosphopeptide - amorphous calcium phosphate and gel with calcium glycerophosphate, surface of the enamel samples of both groups had the signs of an increased mineralization due to the long-term preservation of a layer of additional chemical compounds. Conclusion. The surface of the permanent teeth enamel immediately after the eruption, and during next 12-18 months has visible signs of insufficient mineralization. Under the influence of exogenous means for caries prevention with casein phosphopeptide - amorphous calcium phosphate and calcium glycerophosphate for 12 months visible signs of increased mineralization level of enamel surface appear.
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