Features of VEGF expression in the cervix of uterus in cervical intraepithelial neoplasia associated with human papillomavirus infection in infertility.

Authors

  • E. O. Kindrativ ДВНЗ «Івано-Франківський національний медичний університет», Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2015.2.36-41

Keywords:

vascular endothelial growth factor, cervical intraepithelial neoplasia, infertility

Abstract

Background. The value of vascular endothelial growth factor in neoplastic transformation has a mixed assessment indicating the need to find mechanisms and develop a methodology to assess the structural and functional characteristics of vascularisation, including features of VEGF expression in conditions of cervical intraepithelial neoplasia. Objective. The purpose of investigation was to determine the VEGF expression in the cervix in cervical intraepithelial neoplasia associated with human papillomavirus (HPV) infection in women with disorders of the reproductive function. Methods. 157 biopsies of cervix from women with infertility were investigated. In cervical smears types of HPV with high cancerogenic risk were identified by polymerase chain reaction. We used monoclonal anti-VEGF antibodies. During evaluation of immunohistochemical staining of VEGF semiquantitative method was used with 4 categories: 0 - negative reaction (equivalent to the normal epithelium), 1 - weak coloration (positively stained individual cells, or distributed in fully layers of epithelium, but with weakly expression); 2 - moderate coloration (more amount positively stained cells); 3 - intense colour (stained practically all epithelial cells). Results. The immunohistochemical investigation revealed certain regularity for VEGF expression in cervical biopsies at CIN that associated with HPV in patients with disorders of the reproductive function. In women with infertility and CIN varying degrees of expression of VEGF were observed. Higher levels of VEGF expression intensity was observed in cervix samples, where the highest rates of HPV (with high cancerogenic risk) viral load (> 5 Ig HO / 105) were detected. We have defined the direct correlation of the level of VEGF expression with the degree of severity of dysplastic process and parameters of HPV (with high cancerogenic risk) viral load. Conclusion. The VEGF can be used in practice as an additional criterion of CIN associated with HPV diagnostics in women with disorders of the reproductive function. Use of the VEGF should improve the objectivity of morphological studies, especially in the verification of the degree of dysplastic.

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How to Cite

Kindrativ, E. O. (2015). Features of VEGF expression in the cervix of uterus in cervical intraepithelial neoplasia associated with human papillomavirus infection in infertility. Морфологія / Morphologia / Morfologìâ, 9(2), 36–41. https://doi.org/10.26641/1997-9665.2015.2.36-41

Issue

Vol. 9 No. 2 (2015)

Section

Статті

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