Histological, immunohistochemical and ultrastructural features of the fetal liver morphology under the influence of lead and nanometals.

Authors

  • V. F. Shatorna State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine
  • Yu. O. Belska State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine
  • V. I. Harets State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2016.3.395-402

Keywords:

morphogenesis, fetal liver, bioantagonism, lead acetate, nanosilver, nanogold

Abstract

Background. Silver and gold have a special place among the variety of minerals. However, the morphology liver at histological and ultramicroscopic level under the combined effect of lead acetate and silver and gold citrates have been insufficiently studied. Objective. Identifying of morphological changes in the liver of rats fetuses as a result of combined influence of lead citrate with silver and gold citrates. Methods. The rats were divided into four groups: control group - animals treated with distilled water; group Pb - animals treated with a solution of lead acetate at a dose of 0.05 mg / kg; group Pb + Ag - animals treated with a solution of lead acetate at a dose of 0.05 mg / kg and silver citrate at a dose of 2 mg / kg; group Pb + Au - animals treated with a solution of lead acetate at a dose of 0.05 mg / kg and gold citrate at a dose of 1.5 mg / kg. On the 20th day of pregnancy performed an autopsy, the liver of fetuses was removed and examined its morphological state. Results. In fetal liver of 20th day of embryogenesis identified increased intensity of accumulation of vimentin, accumulation of marker Ki67 was reduced in comparison to the control group. Degenerative changes in hepatocytes and nucleoli disintegration was identified on ultramicroscopic level. Moderate and uniform accumulation of vimentin in liver tissue of the fetuses was revealed in case of the combined injection of lead acetate and silver citrate. Accumulation of marker Ki67 was increased compared to group of lead exposure. Increase of the number of mitochondria and ribosomes was detected on ultramicroscopic level. Under the combined influence of lead acetate and gold citrate the extensive accumulation of vimentin and decreased level of marker Ki67 was identified. Conclusion. Silver citrate on the background of lead exposure showed compensatory properties, that manifested in decreasing of hepatotoxicity caused by lead acetate.

References

  1. Bilec'ka EM, Chekman IS, Onul NM, Kaplunenko VG, Stus VP. [Bioprotective effect of zinc in macro- and nanoaquachelate form on embryonal development of rats in conditions of lead intoxication]. Medical perspectives. 2013;18(2):114–119. Ukrainian.
  2. Campbell JR, Auinger P. The association between blood lead levels and osteoporosis among adults—Results from the Third National Health and Nutrition Examination Survey (NHANES III). Envi-ronmental Health Perspectives. 2007;115(7):1018–1022.
  3. Dovgal' HV. [Morphological changes of rat liver under the influence of lead acetate and in case of correction in the prenatal period]. Ukrainian morphological Almanac. 2014;1:42–44. Ukrainian.
  4. Letfullin RR, Joenathan C, George TF, Zharov VP. Laser-induced explosion of gold nanoparticles: potential role for nanophotothermolysis of cancer. Nanomedicine. 2006;1(4):473-480.
  5. Shatorna VF, Harets VI, Bilec'ka EM, Onul NM, Nef'odova OO, Ostrovs'ka SS, Stepanov SV, Dihno. [Experimental study of modifying influence of nanoaquachelate gold citrate on embryotoxicity of lead acetate in rats]. Medical perspectives. 2014;19(2):12–17. Ukrainian.
  6. Skalnyiy AV, Zalavina SV. [Bioelements and indicators of embryonic mortality of laboratory rats]. Gazette of OSU. 2006;2:78–81. Russian.
  7. El-Mehi AE, Amin SA. Effect of Lead Acetate on the Thyroid Gland of Adult Male Albino Rats and the Possible Protective Role of Zinc Sup-plementation: A Biochemical, Histological and Morphometric Study. Journal of American Science. 2012;8(7):61-71.
  8. Haouas Z, Sallem A, Zidi I, Hichri H, Mzali I, Mehdi M. Hepatotoxic effects of lead acetate in rats: histopathological and cytotoxic studies. J Cytol Histol. 2014;5(5):1-6.
  9. Elgaml SA, Khalil R, Hashish EA, El-Murr A. Protective Effects of Selenium and Alpha-Tocopherol against Lead-Induced Hepatic and Renal Toxicity in Oreochromis Niloticus. Journal of Aquaculture Research & Development. 2015;6:1.
  10. Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gramnegative bacterium Escherichia coli. Applied and environmental microbiology. 2007;6(73):1712-1720.
  11. Potots'ka OYu, Horbunov AO, Tverdokhlib IV, Murashnkina DH, Khripkov IS , Silkina YuV, inventors; State Establishment “Dnipropetrovsk medical academy of the Health Ministry of Ukraine”. Method for measurement of microscopic structures. Ukraine patent UA 51942. 2010 Aug 10. Int. CI. G01N1/00. Ukrainian.
  12. Avtandilov GG. [Introduction to quantitative pathological morphology]. Moscow: Meditsina; 1980. 216 p. Russian.
  13. Sawatzke CL, Solomons CC. Fixation and embedding of small volumes of platelets for transmission electron microscopy. J Clin Pathol. 1980;33(6):600-2.
  14. Mironov AA, Komissarchik YuYa, Mironov VA. Metodyi elektronnoy mikroskopii v biologii i meditsine: Metodicheskoe rukovodstvo. [Electron microscopy methods in biology and medicine : Methodological Guide]. St. Petersburg: Science; 1994. 400 p. Russian.
  15. Zerbino DD. [Svynets': urazhennya sudynnoyi systemy]. Ukrayins'kyy medychnyy chasopys. 2002;2:34–42. Ukrainian.
  16. Hassan EK, Hegazy AM. Histopathological and weight changes of the rat fetal liver of females fed with low protein diet during pregnancy. AAMJ. 2014;2(12):257-272.

Downloads

How to Cite

Shatorna, V. F., Belska, Y. O., & Harets, V. I. (2016). Histological, immunohistochemical and ultrastructural features of the fetal liver morphology under the influence of lead and nanometals. Морфологія / Morphologia / Morfologìâ, 10(3), 395–402. https://doi.org/10.26641/1997-9665.2016.3.395-402

Issue

Vol. 10 No. 3 (2016)

Section

Статті

License

Copyright (c) 2018 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.