Morphological characteristics of heart after induction of therapeutic hypothermia and the introduction of mesenchymal stromal cells in therapy of experimental myocardial infarction.

Authors

  • А. V. Trofimova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
  • N. А. Chizh Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
  • I. V. Belochkina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
  • V. V. Volina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
  • B. P. Sandomirskiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2016.3.288-292

Keywords:

therapeutic hypothermia, mesenchymal stromal cells, experimental myocardial infarction

Abstract

Background. Diseases of cardiovascular system still occupy the first place among the causes of death and disability of population. Currently the new methods for stimulating the regeneration of damaged myocardium are actively developing. It is assumed that the transplantation of mesenchymal stromal cells (MSCs) can promote the replacement of necrotic areas by newly formed cardiomyocytes and stimulate angiogenesis in the ischemic area. In the last decade the method of application of therapeutic hypothermia in order to protect heart and brain cells from oxygen starvation in acute coronary and cerebral insufficiency has become widespread. Objective. The purpose of this work was to study the morphological features of necrosis development and cardiac remodeling in myocardial infarction in rats after the induction of therapeutic hypothermia and injection of MSCs. Methods. The experiment was carried out in 60 white outbred male rats. All the animals were divided into 4 groups after modeling of infarction by ligation of a. coronaries sinistra: 1 – control (myocardial infarction), 2 – myocardial infarction and induction of hypothermia for 60 min, 3 – myocardial infarction and once intravenous injection of allogeneic placental MSCs (concentration 1.2×106cells/ml), 4 – myocardial infarction and combined use of hypothermia and injection of MSCs. Heart tissue for morphological studies was taken on 7th, 14th and 30th days of experiment. Morphometric studies of myocardial infarction area was performed using BioVision 4,0 program. The relative area of connective tissue, the vessel relative area and the number of vessels were assessed. Results. In group 4, in contrast to other groups with myocardial infarction, on 7th day of the experiment the reparative processes in myocardium prevailed over destructive ones. Reparation of myocardium was manifested in the stimulation of angiogenesis, the development of collateral circulation and also in the recovery of contractile elements of cardiomyocytes of the peri-infarction zone. The data of morphometric study in group 4 showed the smallest area of connective-tissue scar on the 30th day of the experiment. Conclusion. Transplantation of MSCs, and especially, combination of therapeutic hypothermia and MSC transplantation lead to a significant improvement in vascularization in the infarcted zone and reduces the connective tissue scar square surface.

References

  1. Sokolov YuM. [New Indications for myocardial revascularization in Modern Cardiology]. Sertse i sudyny. 2012;(2):24–7. Russian.
  2. Schneider A, Popp E, Böttiger BW. Therapeutic hypothermia after cardiac arrest. Emergency Medicine & Critical Care. 2006;2:1–4.
  3. Holzer M, Bernard SA, Hachimi-Idrissi S, Roine RO, Sterz F, Müllner M; Collaborative Group on Induced Hypothermia for Neuroprotection After Cardiac Arrest. Hypothermia for neuroprotection after cardiac arrest: systematic review and individual patient data meta-analysis. Crit Care Med. 2005 Feb;33(2):414-8.
  4. The Hypothermia after Cardiac Arrest Study Group. Mild therapeutic hypothermia to improve the neurologic outcome after cardiac arrest. N Engl J Med. 2002;346(8):549-56.
  5. Noort WA, Feye D, Van Den Akker F, Stecher D, Chamuleau SA, Sluijter JP, Doevendans PA. Mesenchymal stromal cells to treat cardiovascular disease: strategies to improve survival and therapeutic results. Panminerva Med. 2010 Mar;52(1):27-40.
  6. Maureira P, Marie PY, Yu F, Poussier S, Liu Y, Groubatch F, Falanga A, Tran N. Repairing chronic myocardial infarction with autologous mesenchymal stem cells engineered tissue in rat promotes angiogenesis and limits ventricular remodeling. J Biomed Sci. 2012;19: 93.
  7. Lai RC, ChenTS, Lim SK. Mesenchymal stem cell exosome: a novel stem cell-based therapy for cardiovascular disease. Regen Med. 2011 Jul;6(4):481-92. doi: 10.2217/rme.11.35.
  8. Babaevа GG, Chyzh MO, Galchenko SE, Sandomirsky BP, inventors; IPC&C NAS of Ukraine, assignee. Method of modeling of myocardial infarction. Ukrainian patent UA 65535. 2011 Dec 12. Int. Cl. G09B 23/28. Ukrainian.
  9. Shablii V, Kuchma M, Kyryk V, Onishchenko G, Tsupykov O, Klymenko P, Kuchuk O, Gabrielyan A, DomanskiyT, Onishchenko V, Lukash L, Lobyntseva G. Mesenchymal stromal cells from native and cryopreserved human placenta: phenotype, multipotency and in vivo migration potential. Problems of Cryobiology. 2012;22(2):157-60.
  10. Sarkisov DS, Perov YuL, editors. [Microscopic technique: a guide]. Moscow: Meditsina; 1996. 544 p. Russian.
  11. Avtandilov GG. [Medical morphometry]. Moscow: Meditsina; 1990. 384 p. Russian.
  12. Grin VK, Mikhailichenko VY, Gnilorybov AM, Popandopulo AG, Estrin SI. [Cellular cardiomyoplasty in experimental myocardial infarction: a monograph]. Donetsk: Yugo-Vostok; 2014. 248 p. Russian.

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How to Cite

Trofimova А. V., Chizh N. А., Belochkina, I. V., Volina, V. V., & Sandomirskiy, B. P. (2016). Morphological characteristics of heart after induction of therapeutic hypothermia and the introduction of mesenchymal stromal cells in therapy of experimental myocardial infarction. Морфологія / Morphologia / Morfologìâ, 10(3), 288–292. https://doi.org/10.26641/1997-9665.2016.3.288-292

Issue

Vol. 10 No. 3 (2016)

Section

Статті

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