Histochemical study of skeletal muscles in experimental spinal cord blunt injury.

Authors

  • T. Ya. Raskaliei Bogomolets National Medical University, Kyiv, Ukraine
  • V. B. Raskaliei Bogomolets National Medical University, Kyiv, Ukraine
  • L. B. Shobat Bogomolets National Medical University, Kyiv, Ukraine
  • G. O. Gavriliuk-Skiba Bogomolets National Medical University, Kyiv, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2016.3.243-247

Keywords:

spinal cord, vertebral-spinal trauma, ATPase, LDH, neurotrophic control

Abstract

Background. Investigation of causes, frequency, clinical manifestations of spinal cord injury is necessary for the correct medical treatment, proper transport to the hospital, effective rehabilitation of the patient. Objective. To provide morphological characteristics of the adaptive capacities of skeletal muscle tissue in a neurotrophic violations of the spinal cord control after spinal cord injury. Methods. Under the influence of thiopental anesthesia laminectomia was performed with the following blunt trauma of the spinal cord using a "Device for modeling of the blunt tram of the spinal cord". The experimental animals (10 white laboratory rats) were divided into 2 groups. Histochemical study of the quadriceps and tibial muscles of hind rat limbs was performed. Results. In 1 hour after blunt injury next changes were documented: in skeletal muscle – dilatation of interstitial space between myosymplasts by swelling; lack of active movement in the skeletal muscle of the lower extremities by blocking the conduction of nervous impulses in nerve fibers; histochemical changes manifested in the form of migration of PAS-positive component of the sarcolemma in the interstitial space by increasing the permeability of the sarcolemma, reducing the activity of ATPase, increased acid phosphatase and accumulation of lactate dehydrogenase in the sarcolemma, confirming manifestation of degenerative changes in the myosymplast. Conclusion. Even short-term violation of the innervation of skeletal muscles leads to quick disruption of biochemical processes.

References

  1. Polischuk NE, Korzh NA, Phischenko BYa ,authors; Polischuk NE, editor. [Damage to the spine and spinal cord (the mechanisms, clinical features, diagnosis, treatment)]. Kyiv: Knyha plus; 2001. 388 p. Russian.
  2. Vernon L, author. Spinal Cord Medicine: Principles and Practice. New York: Demos Medical Publishing; 2002. 201 p.
  3. Rindenko VG, Zavelya MS, Chernov OL, authors; Rindenko VG, editor. [Vertebral-spinal trauma. Premedical stage: a manual for self-study]. Kharkiv; 2007. 234 p. Ukrainian.
  4. Tsymbaliuk VI, Nevodnik VI, Salkov NN. [Secondary changes in the spinal cord under the influence of prolonged compression trauma of the spine and spinal cord in the cervical region]. Ukrainskyi neirokhirurgichnyi zhurnal. 2013;4:45-55. Ukrainian.
  5. Vasilyeva EM, Bakanov MI. [Biochemical changes in neurological pathology]. Biomed Khim. 2005;51(6):581-602.
  6. Arun PA. Surgical management of spinal cord injury: controversies and consensus. Hoboken, New Jersey: Wiley-Bleckwel; 2007. 272 p.
  7. Geisler FH. Recovery of motor function after spinal-cord injury: a randomized, placebo-controlled trial with GM-I ganglioside. N Engl J Med. 1991 Jun 27;324(26):1829-38.
  8. Bispo dos Santos G, Cristante AF, Macron RM, Inácio de Souza F, Pessoa de Barros Filho TE, Damascene ML. Spinal Cord injury experimental model and motion evaluation protocol in Wistar rats. Acta Ortop Bras. 2011;19(2):87-91.
  9. Raskaley TYa, Chaikovskyu YuB, Raskaley VB, Kozak HI, inventors; National O.Bohomolets medical university, assignee. [Device for simulation of blunt spinal cord injury]. Ukrainian patent UA 97693. 2015 March 25. Int. Cl. А61В8/00. Ukraini-an.
  10. Hubskiy YuI. [Biological chemistry: text-book]. Kyiv, Ternipil: Ukrmedkniga; 2000. 508 p. Ukrainian.
  11. Boikiv DP, Bondarchuk TI, Ivankiv OL, Kobilinska LI, Konik UV, Makarenko TM, Sklyarov OYa, Tomashevska MF, authors; Sklyarov OYa, editor. [Clinical biochemistry]. Kyiv: Meditsina; 2006. 387 p. Ukrainian.
  12. Sklyarov O, Solski Ya, Velikiy M, Fartushok N, Bondarchuk T, Duma D, authors. [Biochemistry of enzymes. Enzymodiagnostics. Enzymopatology. Enzyme therapy.]. Lviv: Quart; 2008. 289 p. Ukrainian.
  13. Hubskiy YuI. [Biological chemistry: text-book ]. Kyiv-Vinnytsya: Nova knyha; 2009. 508 p. Ukrainian.

Downloads

How to Cite

Raskaliei, T. Y., Raskaliei, V. B., Shobat, L. B., & Gavriliuk-Skiba, G. O. (2016). Histochemical study of skeletal muscles in experimental spinal cord blunt injury. Морфологія / Morphologia / Morfologìâ, 10(3), 243–247. https://doi.org/10.26641/1997-9665.2016.3.243-247

Issue

Vol. 10 No. 3 (2016)

Section

Статті

License

Copyright (c) 2018 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.