Expert and diagnostic criteria to identify the time death according to the study Achilles tendons in late postmortem period.

Authors

  • V. A. Povstyaniy State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2016.3.239-242

Keywords:

time of death, autolysis, connective tissue, post-mortem period

Abstract

Background. Еestablishment of time of death is a core issue of forensic medicine. To solve this problem was proposed many different methods. They can provide answers regarding the death in the first 1-3 days post-mortem period. But after 3 days, because of of putrefaction corpse, the answer to this question is very difficult. At this time, has not found a precise criteria for the death of a few days postmortem period. Objective. Aim of the study was to determine the biochemical and biophysical parameters Achilles tendon in postmortem period. Methods. The material for the study was Achilles tendons of 22 corpses of people. For laboratory studies selected tendons only corpses of people, with accurately known period of death. Obtaining fluid from the tendon was performed by mechanical compression of the biological object in the press without homogenizing tissues. This fluid was stored at - 6-8 ° C. The liquid dissolved before use sterile isotonic solution in a ratio of 1: 4. Biochemical studies were performed using standard methods. Determined the amount of uric acid, urea, bilirubin, total protein, albumin, cholesterol, ALT, GGT and alkaline phosphatase. Made fixing Glow Discharge obtained from the Achilles tendon liquid. Results: In the liquid obtained from the Achilles tendon tissue, there is a change of the enzyme and other substances above. Individual enzymes increased in the 2-3 and 7-9 days after death. Total protein, urea and uric acid increased greatly to the 9-th day after decreased sharply. There have been analyzed the relationships identified components. They have significant differences that depend on the time of death. graphs of the number of separate components of the liquid and the intensity of the glow it gives can quickly determine which term death, depending on the type of graph belongs separate sample of fluid. Conclusion. Established by biochemical changes in individual components of fluid from connective tissue, can be used to determine the limitations of death. Assessment of the biological object by registering glow can be used to determine the death in late post-mortem period. For comparative analysis should be used graphic images.

References

  1. Shevchenko I.N. [Stage of the biological process of decomposition of a corpse at different times post-mortem period]. Issues of theory and practice is judicial-medical examination.1995; 69-72. Russian.
  2. Vitter VI, Kulikov VA. [Issues of morphology in solving the problem of determining the prescription of death coming]. A forensic examination. 1999; 3: 3-6. Russia.
  3. Sokolova ZY. [Forensic evaluation of changes in intraocular pressure for establishment of prescription of death coming]. A forensic examination. 2008;3. Russia.
  4. Tenkov АА, Plaksin VО. [A forensic examination of the corpse in late postmortem period]. Kursk: Kursk state medical University; 2005; 535 р. Russia.
  5. Pigolkin YI, Bogomolova IN, Bogomolov DV, Аmanmuradov АH. [The possibility of histomorphometry in forensic medical theory and practice. Morphometric approaches to diagnostics of prescription of the time of death]. Examination problem in medicine. 2001;4 (04);3-7,31-35. Russia.
  7. Povstyaniy VA. Inventor;Povstyaniy VA .Method of obtaining and study of biological fluid from the connective tissue. Ukrainian patent for useful model. UA 109781.2016 sept.09. Int. CL. G01N 33/48. Ukrainian. 7. Glukhova NV, Pesockaya LA, Kuchuk NG. [The development of water express classification system, that based on a database of gas-discharge emission images]. Collection of scientific works of Kharkiv Air Force University. 2015;3(44). Ukrainian.
  8. Аndrea ED, Iain LL, [Biochemistry Changes That Occur after Death: Potential Markers for Determining Post-Mortem Interval]. PLoS One. 2013; 8(11): 82-8.
  9. Andrea ЕD, Iain LL, [Estimation of post-mortem interval using biochemical markers]. Australian Journal of Forensic Sciences. 2014;1:8-26. DOI: 10.1080/00450618.2013.784356.

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How to Cite

Povstyaniy, V. A. (2016). Expert and diagnostic criteria to identify the time death according to the study Achilles tendons in late postmortem period. Морфологія / Morphologia / Morfologìâ, 10(3), 239–242. https://doi.org/10.26641/1997-9665.2016.3.239-242

Issue

Vol. 10 No. 3 (2016)

Section

Статті

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