Immunohistochemical features of fat tissue of epiploon and subcutaneous fat tissue in patients with overweight, obesity and metabolic syndrome.

Authors

  • K. M. Militsa State Institution “Zaporizhia Medical Academy of Post-Graduate Education Ministry of Health of Ukraine”, Ukraine
  • I. V. Sorokina Kharkiv National Medical University, Ukraine
  • M. S. Myroshnychenko Kharkiv National Medical University, Ukraine
  • O. N. Pliten Kharkiv National Medical University, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2016.3.203-207

Keywords:

fat tissue, epiploon, subcutaneous fat tissue, оverweight, obesity, metabolic syndrome, immunohistochemistry

Abstract

Background. Fat tissue plays a key role in the development of metabolic syndrome determining the necessity of its complex morphological research. Objective: to identify the immunohistochemical features of fat tissue of epiploon and subcutaneous fat tissue in patients with metabolic syndrome and overweight or obesity of I–III degree. Methods. The material of the study was the autopsy and operational material – fat tissue of epiploon and subcutaneous fat tissue. The authors used histological method for review microscopy and immunohistochemical methods with monoclonal antibodies to the tumor necrosis factor alpha (TNF-α), interleukine-6 (IL-6), insulin and cortisol. Results. It was revealed that in people with a normal body mass index, metabolic syndrome and overweight or obesity in epiploon fat tissue comparing with subcutaneous fat tissue the number of cells expressing receptors for TNF-α, IL-6 and cortisol is significantly greater; in subcutaneous fat tissue the number of cells expressing receptors for insulin is significantly greater. In patients with metabolic syndrome with increased body mass index in subcutaneous fat tissue and epiploon the number of cells expressing receptors for TNF-α and IL-6 is increased, the ability to express adipocyte insulin receptors is reduced while the ability to express receptors to cortisol is increased. Conclusion. Taken together these results evidence the presence of immunohistochemical features of fat tissue of epiploon and subcutaneous fat tissue in patients with metabolic syndrome and overweight or obesity.

References

  1. Kolopkova TA, Blinova VV, Skvortsov YI, Subbotina VG. [Metabolic syndrome X – a pandemic of the XXI century]. Saratov Journal of Medical Science. 2008; 3 (21): 130-4. Russian.
  2. Smirnova LE, Vinogradov VF, Smirnov AV, Kovtunova NP, Katchalov AS. [Metabolic syndrome from the viewpoint of a cardiologist and gastroenterologist: modern aspects of the problem (review)]. Verkhnevolzhskiy medical journal. 2012; 10(2): 12-7. Russian.
  3. Egorov AD, Penkov DN, Tkachuk VA. [Molecular and cellular mechanisms of adipogenesis]. Diabetes mellitus. 2015; 18 (2): 12-9. Russian.
  4. Sveklina TS, Talantseva MS, Barsukov AV. [The metabolic syndrome and inflammation: topical issues of pathogenesis]. Clinical laboratory diagnostics. 2013; 3: 7-10. Russian.
  5. Butrova SA, Ershov EV, Ilyin AV. [Adipocytokines: resistin and tumor necrosis factor alpha in men with abdominal obesity]. Obesity and metabolism. 2007; 4: 30-3. Russian.
  6. Shkolnik VV, Shaposhnikova YN, Nemtsov VD. [The relationship of ghrelin and TNF-A with various components of the metabolic syndrome]. Zaporozhye medical journal. 2012; 2 (71): 78-84. Russian.
  7. Hoene M, Weigert C. The role of interleukin-6 in insulin resistance, body fat distribution and energy balance. Obesity reviews. 2008; 9: 20-9.
  8. Dedov II, Melnichenko GA, Butrova SA. [Adipose tissue as an endocrine organ]. Obesity and metabolism. 2006; 1: 6-13. Russian.
  9. Nikonova LV, Tishkovsky SV, Gulinskaya OV, Doroshkevich IP, Janets NV, Davydchik EV. [The metabolic activity of adipose tissue and its role in the formation of insulin resistance]. Journal of Grodno State Medical University. 2012; 1: 7–9. Russian.
  10. Chuchelina OA. [Adipokines of adipose tissue and their role in the progression of renal pathology]. International journal of medicine. 2015; 2: 24-8. Russian.
  11. Ivashkin VT, Maevskaya MV. [Lipotoxicity and metabolic disorders in obesity]. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2010; 20 (1): 4-13. Russian.
  12. Chernyshova AL, Kolomiets LA, Bochkareva NV, Asadchikova ON. [Metabolic syndrome, the relationship with the processes of carcin-ogenesis of endometrium]. Siberian journal of oncology. 2008; 5 (29): 68-74. Russian.
  13. Chubrieva SYu, Glukhov NV, Zaichik AM. [Adipose tissue as an endocrine regulator (review)]. Bulletin of St Petersburg State University. 2008; 11 (1): 32-43. Russian.

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How to Cite

Militsa, K. M., Sorokina, I. V., Myroshnychenko, M. S., & Pliten, O. N. (2016). Immunohistochemical features of fat tissue of epiploon and subcutaneous fat tissue in patients with overweight, obesity and metabolic syndrome. Морфологія / Morphologia / Morfologìâ, 10(3), 203–207. https://doi.org/10.26641/1997-9665.2016.3.203-207

Issue

Vol. 10 No. 3 (2016)

Section

Статті

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