The effect of ademetionine on morphological changes in the kidneys of rats under experimental rhabdomyolysis.
DOI:
https://doi.org/10.26641/1997-9665.2016.3.161-164Keywords:
rhabdomyolytic acute kidney injury, ademetionine, morphological changes, renoprotectionAbstract
Background. It is estimated that rhabdomyolysis is the cause of 5–15% of acute kidney injury (AKI) cases. At present days timely prophylactic correction of AKI is the key stone of nephrology and pharmacological research. Ademetionine has the antioxidant and cytoprotective properties suggesting the renoprotective potential under the conditions of AKI. Objective. To study the kidney morphology under the conditions of rhabdomyolytic AKI and its correction by ademetionine. Methods. The study was conducted on 28 non-linear male rats. Rhabdomyolytic AKI was induced by intramuscular injection of 50% glycerol (10 ml/kg). Ademetionine (40 mg/kg, “Heptral”, Abbott, Italy) was injected 40 min after glycerol administration. 24 h after glycerol administration kidney samples were collected. Documentation of the pathological processes was performed by the computer morphometry of objects in histological preparations. Results. Histopathological kidney examination revealed the morphological changes in the renal cortex and medulla manifested by luminal obstruction of the cortical (28±0.8%) and medullary (61±1.2%) tubules with myoglobin casts, hydropic and granular dystrophic changes of tubular epithelial cells (94±1.0%) and signs of coagulative necrosis in the proximal tubular cells (2±0.1%). Use of ademetionine significantly improved renal histology confirmed by restriction of dystrophy (66±0.8% of proximal tubular cells), necrotic processes (1.7±0.2%) and significant reduction of luminal obstruction by myoglobin casts (0.4±0.4% of cortical and 1.2±0.1% of medullary tubules), indicating on its nephoprotective potential. Conclusion. Ademetionine demonstrates the renoprotective activity under the conditions of rhabdomyolysis-induced kidney injury by prevention of the significant histopathological changes in kidney tissue.
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