The morphological transformations of the rat tongue under the conditions of general dehydration.

Authors

  • L. N. Davydova Sumy State University, Ukraine
  • G. F. Tkach Sumy State University, Ukraine
  • V. Z. Sikora Sumy State University, Ukraine
  • O. S. Maksymova Sumy State University, Ukraine
  • D. V. Muravskyi Sumy State University, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2016.3.118-123

Keywords:

the mucous membrane of tongue, dehydration, ultrastructure

Abstract

Background. The pathology of water and electrolyte balance is quite often observed in clinical practice, while it may hinder or disrupt the structural and functional state of the human tongue. The objective is to identify the features of rat's tongue morphological changes under severe degree of general dehydration at the microscopic and ultrastructural levels. Methods. The experiment was performed on 20 white laboratory rats. The animals were divided into experimental and control group (10 rats respectively). A severe degree of general dehydration was modeled by A.D.Soboleva for rats from the experimental series. In the work investigation of the next morphometrical parameters was performed: papilla length, diameter of base and apex of papilla, diameter of taste bud, thickness of epithelium and the thickness of the keratinized layer, lamina propria thickness, keratinization index, mitotic index, the nuclear-cytoplasmic ratio of epithelial cells, diameter of arteriole, diameter of capillary, diameter of venule, arteriolar-venular coefficient, diameter of muscle fiber, width of endomysium, width of perimysium, the square surface of nucleus, the square surface of mitochondria, the volume of nucleus, the volume of mitochondria. Results. The impact of the severe degree of general dehydration has led to the reduction of epithelium thickness for 19,31 % (р = 0,0001), lamina propria thickness was reduced for 31,89 % (р = 0,0001), and also led to increase of the horny layer thickness of the epithelium for 11,75 % (р = 0,0007). The keratinization index increased regarding the benchmark for 38,34 % (р = 0,0001). The nuclear-cytoplasmic ratio of basal cells increased for 23,89 % (р = 0,0001), of spinous cells increased for 27,76 % (р = 0,0479) and of granulosa cells increased for 31,37 % (р = 0,027). The arteriolar-venular coefficient increased for 5,51 % (р = 0,1851). The diameter of muscle fiber reduced for 27,93 % (р = 0,0009), the width of endomysium reduced for 24,31 % (р = 0,048) and the width of perimysium – for 22,73 % (р = 0,0486). The diameter of myofibril and the volume of myosymplast nucleus reduced for 8,56 % (р = 0,0064) and 19,35 % (р = 0,028) respectively. The arteriole diameter, capillary diameter, venule diameter of tongue muscle reduced for 23,91 % (р = 0,0001), 41,26 % (р = 0,0003), 27,53 % (р = 0,0001) respectively. Conclusion. Severe degree of general dehydration leads to the atrophic changes in the epithelial cells of the tongue mucosa and disruption of keratinization process. Furthermore, it leads to the loss of regularity in cross-striation, excessive reduction of myofibrils, hypertrophic and destructive changes in the mitochondria of tongue muscle. These changes occur on the background of organ microcirculation disorders manifested by a significant narrowing of the vessel lumen, capillaries stasis and perivascular hemorrhages.

References

  1. Yeroshenko GA, Semenova AK, Gasiuk NV. [Prevalence and structure of diseases tongue in young patients]. World of medicine and biology. 2013; (3 Pt 2):14-6. Ukrainian.
  2. Lutskaya IK. [Symptomatic and independent disease of tongue]. Meditsinskie novosti. 2015; 3(246):13-17. Russian.
  3. Sokolova OA, Avanesov AM. [Changes in the mucous membrane of oral pathology in the gastrointestinal tract]. Electronic Research and Education Gazette "Health and Education in the XXI Century". 2009; (5):216-7. Russian.
  4. Zalavina SV, Apraksina EY, Pushilin PI, Zhelezny SP, Pushilina MY, Elyasin PA. [Morphofunctional features of mucous membrane of oral cavity against vibrating influence]. Medicine and education in Siberia. 2014; 6:39. Russian.
  5. Gazhva SI, Zagaynov VE, Igolkina NA, Lipatov KS, Murtazalieva MS. [Effects of chronic kidney disease on the oral mucosa]. Modern problems of science and education. 2013;(2):2. Russian.
  6. Mitsudo K, Kobayashi M, Tohnai I, Ueda M, Kotani H, Hoshino T. Electron-microscopic and immunohistochemical studies of Langerhans cells and Thy-1-positive cells in mouse tongue epithelium subjected to local hyperthermia. Arch Oral Biol. 1995 Jun;40(6):533-8. doi:10.1016/0003-9969(94)00194-G.
  7. Hooper L, Bunn D, Jimoh FO, Fairweather-Tait SJ. Water-loss dehydration and aging. Mech Ageing Dev. 2014 Mar-Apr;136-137:50-8. doi: 10.1016/j.mad.2013.11.009.
  8. Soboleva AD. [Reaction of cells and tissues in the dehydration]. Novosibirsk: Nauka; 1975. 64 p. Russian.

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How to Cite

Davydova, L. N., Tkach, G. F., Sikora, V. Z., Maksymova, O. S., & Muravskyi, D. V. (2016). The morphological transformations of the rat tongue under the conditions of general dehydration. Морфологія / Morphologia / Morfologìâ, 10(3), 118–123. https://doi.org/10.26641/1997-9665.2016.3.118-123

Issue

Vol. 10 No. 3 (2016)

Section

Статті

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