Morphometric changes in Bcl-2+-cells of the parietal lobe cortex induced by incomplete global cerebral ischemia-reperfusion in male rats with streptozotocin-induced diabetes mellitus.

Authors

  • T. M. Boychuk Bukovinian State Medical University, Chernivtsi, Ukraine
  • T. I. Kmet Bukovinian State Medical University, Chernivtsi, Ukraine
  • O. G. Kmet Bukovinian State Medical University, Chernivtsi, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2016.3.63-66

Keywords:

diabetes mellitus, carotid ischemia-reperfusion, Bcl-2 nerve and glial cells

Abstract

Background. Trigger mechanisms causing death of the nerve and glial cells in the cerebral cortex by means of apoptosis under conditions of diabetes mellitus and ischemia-reperfusion are energy deficiency, ion imbalance etc. Although, certain protective mechanisms resist them, including apoptosis and antiapoptotic Bcl-2 protein in particular.       Objective of the study is to find the peculiarities of morphometric changes in Bcl-2 nerve and glial cells of the parietal lobe of cerebral cortex in during incomplete global ischemia-reperfusion in male rats with experimental diabetes. Methods. The nerve and glial cells of the parietal lobe of cerebral cortex positive for Bcl-2 protein were identified by means of immunofluorescent method with the videocamera COHU-4722. In the obtained images the computer system of digital analysis VIDAS-386 determined morphometric parameters of Bcl-2+ cells, in particular: their square surface, coefficients of the shape and elongation. Results and conclusion.        The results of the experimental study indicated the fact that in animals without diabetes mellitus 20-minute bilateral carotid ischemia with 1 hour reperfusion modifies the coefficient of the shape and elongation of Bcl-2+ nerve cells and the coefficient of the shape of glial cells. On the 12th day of the study the indicated changes of Bcl-2+ nerve and glial cells remains and are supplemented with changes in the square surface of the given cells. In animals with diabetes mellitus the square surface of Bcl-2+ nerve cells of the parietal lobe of cerebral cortex decreases in the early ischemic-reperfusion period, and on the 12th day changes are found in all the examined morphometric parameters of the nerve cells. In both periods of the study changes of the morphometric parameters in glial cells are not found.

References

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How to Cite

Boychuk, T. M., Kmet, T. I., & Kmet, O. G. (2016). Morphometric changes in Bcl-2+-cells of the parietal lobe cortex induced by incomplete global cerebral ischemia-reperfusion in male rats with streptozotocin-induced diabetes mellitus. Морфологія / Morphologia / Morfologìâ, 10(3), 63–66. https://doi.org/10.26641/1997-9665.2016.3.63-66

Issue

Vol. 10 No. 3 (2016)

Section

Статті

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