Microscopic alterations of the thymus under the damaging influence of experimental dehydration.

Authors

  • O. О. Prykhodko Sumy State University, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2017.2.35-39

Keywords:

thymus, accidental transformation, dehydration, rats.

Abstract

Background. Thymus is the central organ of immune system and is extremely sensitive to pathological exogenous factors which trigger its involution. Objective. To establish the features of thymus morphological changes in conditions of mild and total organism dehydration in an experiment. Methods. The study was conducted on 24 white mature male rats. Animals were kept on anhydrous diet for 3 and 6 days thus dehydration was simulated. For the structural components investigation, histological sections were stained with hematoxylin and eosin, methylene blue, and by Van-Gizon. The square surface ratio of medulla, cortex and stromal component of the thymus was calculated by the system of computer analyses "Digimaizer". Measurements were carried out in 10 areas of thymus histological section of each rat. Throughout present research it was determined the number of lymphocytes in the area of vision on the surface 0.009 mm2 (magnification ×1000) in the cortex and medulla using the program "ImageJ". Results. During the investigation morphological structure of the control group thymus is not distinguished from the one described in the articles of researchers and histology tutorials, devoted to the thymus structure. In the experimental groups it was established that dehydration qualitatively changes the structure of the thymus. While under mild thymus dehydration in most observations detected signs of the II phase of accidental transformation, were characterized by focal delymphatisation of the cortex of thymic lobules. During moderate degree of dehydration, elements of the II, III і IV phases of accidental transformation occurred where in the prevailed observations detected signs of III phase of the accidental transformation: the boundary between the cortex and medulla is not defined in thymus lobules. The II phase of accidental transformation in the most of observations appears on the grounds of follicular hyperplasia. During dehydration the content of connective tissue component was increased and the number of lymphocytes in the thymus was reduced. Conclusion. Increasing degree of overall dehydration enhance the manifestations of accidental thymus transformation.

References

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How to Cite

Prykhodko O. О. (2017). Microscopic alterations of the thymus under the damaging influence of experimental dehydration. Морфологія / Morphologia / Morfologìâ, 11(2), 35–39. https://doi.org/10.26641/1997-9665.2017.2.35-39

Issue

Vol. 11 No. 2 (2017)

Section

Статті

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