Investigation methods of adhesion of microorganisms.

Authors

  • N. M. Semenko State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine
  • D. O. Stepanskiy State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine
  • N. G. Smotrova State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine
  • I. Yu. Stetsenko State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine
  • A. M. Ivanova State institution “Dnipropetrovsk medical academy of the Ministry of Health of Ukraine”, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2016.4.7-11

Keywords:

adhesion, virulence, photocolorimetry, diagnostics of infectious diseases

Abstract

Background. Adhesion is the main property of microorganisms that determines its pathogenicity. Laboratory examination of adhesion is important to determine the virulence of the bacteria and its effect on the development of pathology. Objective. Compilation of the main research methods of microbal adhesion and their possible use in common laboratory diagnostics. Results. Microbal adhesion is explored in vivo and in vitro. Research in vivo requires the native biological models. For example, studies of adhesion of S. aureus relatively a rats’ blood vessels when fluorochrome labeled bacteria enter the body of biological models and watch the number of microorganisms adherent to the arterial walls of the peritoneum, previously performed surgical access. The scientists used fluorescent microscopy for this. Research methods of microbal adhesion in vitro include the study of the adhesive properties of red blood cells using Group 1 (O) Rh +; research using adhesive properties of mammals’ red blood cells; study the microbal adhesion to hemoprotein, fibronectin and fibrinogen; evaluation of adhesive properties by Photocolorimetry; measuring forces acting between S. aureus and human skin. Conclusion. The most accurate and easy-to-carrying is a research method of adhesion as Photocolorimetry. It’s very important to use the definition of microorganism adhesive properties not only for scientific purpose, but also in usual diagnostics of infectious diseases.

References

  1. Shyrobokov VP, editor. Medychna mikrobiologiya, virusologiya I imunologiya: pidruchnik dlya studentiv vyshchih navchalnih zakladiv [Medical Microbiology, Virology and Immunology: textbook for students in higher medical education]. Vinnitsa: Nova knyha; 2010. 952 p. Ukrainian.
  2. Pimenov EV, Oborin VA, Ivonin AG. [Evaluation of adhesion properties of Bacillus anthracis in the red blood cells of mammals using photocolorimetry]. Problemy osobo opasnyh infektsiy. 2011;4:41-43. Russian.
  3. Claes J, Liesenborghs L, Lox M, Verhamme P, Vanassche T, Peetermans M. In vitro and in vivo model to study bacterial adhesion to the vessel wall under flow conditions. J Vis Exp. 2015 Jun 11;(100):e52862. doi: 10.3791/52862.
  4. Pavlova IB, Lenchenko EM, Shtukareva MYu, Belousov VI. [Methodic recommendations for the preparation the preparations for the study of the degree of microbal adhesion to animal cells in a light and scanning electron microscope]. Ministry of Agriculture of Russian Federation. 1999;13(7):2-4. Russian.
  5. Formosa-Dague C, Fu ZH, Feuillie C, Derclaye S, Foster TJ, Geoghegan JA, Dufrene YF. Forces between Staphylococcus aureus and human skin]. Nanoscale Horizons. 2016;1:298-303. DOI: 10.1039/C6NH00057F.
  6. Romanov VE, Ivonin AG, Bondarenko AL, Oborin VA, Nehoroshkina EL, inventors; Federal State Institution of Higher Education “Vyatka State Agricultural Academy”, assignee. [The method for determining the activity of erythrocyte fixing bacteria]. Russian patent. RU 2360969. 2009 Jul 10. Int CL C12Q1/02. Russian.
  7. Naumenko ZS, Shpicina IV. [Comparative evaluation of adhesion activity of bacteria isolated from patients with osteomyelitis hearth and of the open fracture wounds]. Heniy ortopedii. 2011;4:31-34. Russian.
  8. Potaturkina-Nesterova NI, Falova OE, Volgina TI, inventors; Federal State Educational Institution of Higher Professional Education “Ulyanovsk State Technical University”, assignee. [The method for determining the adhesion of Staphylococcus aureus]. Russian patent. RU 2510024. 2012 Apr 4. Int CL C12Q1/14. Russian.
  9. Seregina NV, Salomatina TV. [Study of adhesive ability listeria monocytogenes to human erythrocytes and sheep]. In: [Contemporary medicine: current issues; 2013 Nov 28; Novosibirsk, Russia]. SibAK; 2013. p. 108-115. Russian.
  10. Tyurin YuA, Mustafin IG, Fassahov RS, inventors; Federal State Institution of Science "Kazan Research Institute of Epidemiology and Microbiology" of Rospotrebnadzor, State Educational Institution of Higher Professional Education "Kazan State Medical University, Federal Agency for Healthcare and Social Development", assignee. [A method for determining the adhesion of Staphylococcus spp. to hemoprotein]. Russian patent. RU 2393229. 2010 Jun 27. Int CL C12Q1/14. Russian.
  11. Tyurin YuA, Fassahov RS, inventors; Federal State Institution of Science "Kazan Research Institute of Epidemiology and Microbiology" of Rospotrebnadzor, assignee. [A method for determining the adhesion of Staphylococcus spp. to fibronectin and fibrinogen]. Russian patent. RU 2490329. 2013 Aug 20. Int CL C12Q1/14. Rus-sian.

Downloads

How to Cite

Semenko, N. M., Stepanskiy, D. O., Smotrova, N. G., Stetsenko, I. Y., & Ivanova, A. M. (2016). Investigation methods of adhesion of microorganisms. Морфологія / Morphologia / Morfologìâ, 10(4), 7–11. https://doi.org/10.26641/1997-9665.2016.4.7-11

Issue

Vol. 10 No. 4 (2016)

Section

Статті

License

Copyright (c) 2018 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.