Complex immunohistochemical analysis of diffuse gliomas of the brain.

Authors

  • І. S. Shpon‘ka State establishment “Dnipropetrovsk medical academy of Health Ministry of Ukraine”, Ukraine
  • T. V. Shynkarenko State establishment “Dnipropetrovsk medical academy of Health Ministry of Ukraine”, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2017.3.39-46

Keywords:

diffuse glioma, immunohistochemistry, diagnostics, proliferation index, angiogenesis, intermediate filament, matrix metalloproteinase.

Abstract

Background. Differential diagnostics of diffuse gliomas is complicated by the morphological similarity of tumors that have a different malignant potential. Objective. Therefore, the aim of this study was to optimize the diagnostic morphological criteria of glial brain tumors on the basis of studying the immunohistochemical expression of markers of biological properties of cells. Methods. Range of primary antibodies (Ki-67, EGFR, p53, MGMT, MMP3, MMP9, GFAP, vimentin, cytokeratin AE1/AE3, IDH1 R132H) was used for IHC. Contingency tables were analyzed with Fisher's exact test. Spearmen rank coefficient was computed to identify a relationship between morphometric parameters and tumor Grade. Results. Authors have analyzed cell kinetics, apoptosis, DNA repair, proteolysis of extracellular matrix, metabolic disorders, and changes in the expression of intermediate filaments, angiogenesis. Immunophenotypes were defined for adult glial tumors (Grade II, Grade III and Grade IV; astrocytic and oligodendroglial gliomas). Morphological differential criteria were proposed. Conclusion. Immunophenotype characteristics of tumors of different histological type and WHO Grade were determined.

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How to Cite

Shpon‘ka І. S., & Shynkarenko, T. V. (2017). Complex immunohistochemical analysis of diffuse gliomas of the brain. Морфологія / Morphologia / Morfologìâ, 11(3), 39–46. https://doi.org/10.26641/1997-9665.2017.3.39-46

Issue

Vol. 11 No. 3 (2017)

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