Ultramicroscopic changes of rat parathyroid glands after immunosuppression

Authors

  • V. V. Erokhina Kharkiv National Medical University, Ukraine
  • O. V. Avilova Kharkiv National Medical University, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2017.4.35-39

Keywords:

parathyroid gland, rats, ultrastructure, parathyroid cell, cyclophosphamide

Abstract

Background. Parathyroid glands actively respond to exogenous and endogenous factors that confirmed by a number of fundamental researches. Currently there are no comprehensive morphological works devoted to the study of ultramicroscopic features of parathyroid glands during immune status changing. Cyclophosphamide is widely used in the clinical practice. It’s belongs to a class of drugs known as alkylating agents, which have been used to treat some types of cancer. Objective. The objective of the work was to study features of electron-microscopic structure of the rat parathyroid glands under immunosuppression caused by the administration of cyclophosphamide. Methods. The study was conducted on 24 outbred male mature rats with the weight 180±10g. Maintenance and care of animals were conducted in according to the norms of bioethics. The animals received an intramuscular injection of cyclophosphamide in a dose of 200 mg/kg body weight. The animals were withdrawn from the experiment on the 7th and 30th day after the injection. The ultrastructure of rat parathyroid glands in normal condition as well as after immunosuppression was studied. Results. The parenchyma of the parathyroid glands of intact animals is composed of two cell types: chief cells and oxyphil cells. Principal (chief) cells are the most numerous type of the gland’s parenchyma. They are small, polygonal cells, with a centrally located nucleus. The cytoplasm of the chief cells contains well-developed rough endoplasmic reticulum and Golgi apparatus. There are numerous dense secretory vesicles, large accumulations of glycogen, and lipid droplets in the cytoplasm. Oxyphil cells constitute a minor portion of the parenchymal cells. The cells are more rounded, considerably larger than the principal cells. Mitochondria, often with bizarre shapes and sizes, almost fill the cytoplasm and are responsible for the strong acidophilia of these cells. No secretory vesicles and little if any rough endoplasmic reticulum are present. Cytoplasmic inclusion bodies consist of occasional lysosomes, lipid droplets, and glycogen distributed among the mitochondria. Conclusion. The introduction of cyclophosphamide to rats in high doses caused marked changes in the ultrastructure of the organ. Polymorphism of cell nuclei, reducing the amount of the organelles that are participate in the protein synthesis, reducing the number of secretory granules, increasing the number of lipid and colloid droplets in the cell cytoplasm were observed in the early period of observation (7th day). On the 30th day after the administration of cyclophosphamide tendency to normalization of morphological structure of the organ revealed.

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Published

2017-12-22

How to Cite

Erokhina, V. V., & Avilova, O. V. (2017). Ultramicroscopic changes of rat parathyroid glands after immunosuppression. Морфологія / Morphologia / Morfologìâ, 11(4), 35–39. https://doi.org/10.26641/1997-9665.2017.4.35-39

Issue

Vol. 11 No. 4 (2017)

Section

Статті

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